| Service | Setup Fee | Per Sample | Setup Fee | Per Sample | Setup Fee | Per Sample | Samples per Setup Fee |
|---|---|---|---|---|---|---|---|
| Agilent 4200 TapeStation D5k | $17.92 | $7.40 | $20.61 | $8.51 | $23.30 | $9.62 | 15 |
| Agilent TapeStation D1000 | $18.09 | $7.48 | $20.81 | $8.60 | $23.52 | $9.72 | 15 |
| Agilent TapeStation Genomic DNA | $19.62 | $9.35 | $22.57 | $10.75 | $25.51 | $12.16 | 15 |
| Agilent TapeStation High Sensitivity D1000 | $20.87 | $9.15 | $24.00 | $10.52 | $27.13 | $11.89 | 15 |
| Agilent TapeStation High Sensitivity D5000 | $20.84 | $10.35 | $23.97 | $11.90 | $27.09 | $13.45 | 15 |
| Agilent TapeStation High Sensitivity RNA | $20.57 | $7.60 | $23.66 | $8.74 | $26.74 | $9.88 | 15 |
| Agilent TapeStation RNA | $19.40 | $7.07 | $22.31 | $8.13 | $25.22 | $9.19 | 15 |
| Agilent BioAnalyzer - DNA High Sensitivity | $155.77 | $4.64 | $179.14 | $5.34 | $202.50 | $6.03 | 11 |
| Agilent BioAnalyzer - RNA Nano | $117.98 | $4.64 | $135.68 | $5.34 | $153.37 | $6.03 | 12 |
| Agilent BioAnalyzer - RNA Pico | $122.42 | $4.64 | $140.78 | $5.34 | $159.14 | $6.03 | 11 |
| Agilent BioAnalyzer - Small RNA | $141.32 | $4.64 | $162.51 | $5.34 | $183.71 | $6.03 | 11 |
Bioanalyzer & TapeStation
The BPF is equipped with an Agilent Bioanalyzer and an Agilent TapeStation. Both instruments can analyze DNA and RNA. These multipurpose platforms are designed as microfluidic alternatives to gel electrophoresis. The "lab-on-a-chip" technology integrates sample separation with automated analysis to provide reproducible data faster and more economically than traditional methods. Results can be viewed as an electropherogram or as a standard gel-like image. For more details about the Agilent systems please see their brochures.
The Bioanalyzer and TapeStation have the ability to determine the quantity and quality of Total RNA, mRNA, cDNA, cRNA, ssDNA as well as dsDNA. These technologies can also be used to evaluate Next-Gen libraries. There are a variety of LabChips and ScreenTapes to choose from depending on the type and size range of your samples. The Boanalyzer utilizes a 25bp marker to standardize each lane to the ladder that is run with each chip type. The marker and the ladder aid in determining the size and quality of the sample. The TapeStation includes a ladder to be run with each ScreenTape to perform similar analyses. You will receive a PDF file as well as an instrument software specific file (.D1K, .D5K, .R6K, etc for the TapeStation, .xad for the Bioanalyzer). You must download Agilent software to view and manipulate the instrument specific files. Links to the downloads are available in our Data Analysis section.
- Agilent Bioanalyzer
- Agilent TapeStation
Please bring your order form and samples to the Biopolymers Facility located at NRB Room 0088 (9:00 am - 5:00 pm, Monday - Friday).
All samples should meet the following criteria:
- Provide at least 2uL of each sample.
- Samples must meet concentration criteria, listed below. Please dilute your samples if they are out of range.
- Submit samples in a 0.2mL PCR tube.
- Label tubes with sample name.
- Samples should be on dry ice.
| Instrument | Chip or ScreenTape | Nucleic Acid Type | Concentration Range |
|---|---|---|---|
| Agilent TapeStation | D1000 | DNA | 0.1 ng/ul to 50 ng/ul |
| Agilent TapeStation | D5000 | DNA | 0.1 ng/ul to 50 ng/ul |
| Agilent TapeStation | Genomic | DNA | 10 ng/ul to 100 ng/ul |
| Agilent TapeStation | High Sensitivity D1000 | DNA | 10 pg/ul to 1000 pg/ul |
| Agilent TapeStation | High Sensitivity D5000 | DNA | 10 pg/ul to 1000 pg/ul |
| Agilent TapeStation | High Sensitivity RNA | RNA | 500 pg/ul to 10000 pg/ul |
| Agilent TapeStation | RNA | RNA | 25 ng/ul to 500 ng/ul |
| Agilent BioAnalyzer | DNA High Sensitivity | DNA | 5 pg/ul to 500 pg/ul |
| Agilent BioAnalyzer | RNA Nano | Total RNA | 5 ng/ul to 500 ng/ul |
| Agilent BioAnalyzer | RNA 6000 Nano | mRNA | 25 ng/ul to 250 ng/ul |
| Agilent BioAnalyzer | RNA Pico | Total RNA | 50 pg/ul to 5000 pg/ul |
| Agilent BioAnalyzer | RNA 6000 Pico | mRNA | 250 pg/ul to 5000 pg/ul |
| Agilent BioAnalyzer | Small RNA | small RNA* | 50 pg/ul to 2000 pg/ul |
Services
For additional protocols and documentation, please visit the Agilent website http://www.agilent.com/.
We review the traces from each LabChip or ScreenTape to look for run anomalies and will repeat samples that have poor data as a result of an instrument or reagent problem. Otherwise, it is your responsibility to evaluate the data for quality and concentration. For RNA a RNA Integrity Number (RIN) value (Bioanalyzer) or RNA Integrity Number Equivalent (RINe) value (TapeStation)is generated to represent RNA quality. More details regarding these values can be found on the Agilent website. You must download Agilent software to view and manipulate the instrument specific files